LRIG1 is a gatekeeper to exit from quiescence in adult neural stem cells

Abstract Adult neural stem cells (NSCs) must tightly regulate quiescence and proliferation. Single-cell analysis has suggested a continuum of cell states as NSCs exit quiescence. Here we capture characterize in vitro primed quiescent identify LRIG1 an important regulator. We show that BMP-4 signaling induces dormant non-cycling state (d-qNSCs), whereas combined BMP-4/FGF-2 distinct poised for cycle re-entry. Primed (p-qNSCs) are defined by high levels CD9, well interferon response signature, can efficiently engraft into the adult subventricular zone (SVZ) niche. Genetic disruption Lrig1 vivo within SVZ leads enhanced Mechanistically, primes re-entry EGFR responsiveness enabling protein to increase but limiting activation. is therefore functional regulator NSC from